regulating notch meaning in Chinese
调整挡[凹口
Examples
- To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei , and to study the function and mechanism of mint - mediated transcription repression , yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5 , amino acids 2226 - 2959 ) . from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo , fifty - one were positive for nutritional screening and p - galactosidase assay . restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments , which are mint , alpha a - crystallin - binding protein i ( alphaa - crybpl ) , and nuclear receptor co - repressor 1 ( n - corl ) , respectively
鉴于mwt基因编码区较长，共有10799个碱基，故此我们将mint分为六段，分别命名为fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段为研究对象，将h者分别插入pgb盯7载体中，结果显示： mintfs可与核受体辅助抑制因子1阶cori ） ， o晶状体蛋白结合蛋白1hlphatcrybp入及mw加互作用，而f6可与igm的重链恒定区、泛素结合酶2l6和一个未知功能的新的蛋白基因进行结合。